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歷 年 稿 件 內 容
 
*類別：
* 姓名： 陳怡伶
投稿種類： 口頭
*中文投稿標題： Next generation sequencing to analyze T-cell receptor clonalities in T-cell Lymphoma in Taiwan
*中文作者姓名列：
*中文服務單位：
*英文投稿標題：
*英文作者姓名列：
*英文服務單位：
* 投稿摘要： Lymphomas are a heterogeneous group of diseases caused by accumulation of malignant cells in the lymphoid tissues. Diagnosis of lymphoid neoplasm sometimes is challenging. Both histopathologic criteria and immunohistochemical characterization of candidate cells are usually employed by pathologists to make an accurate diagnosis. In cases with atypical histopathological features, however, clonality assessment of T-cell receptor (TCR) and immunoglobulin (Ig) provides indispensible information in the differentiation of lymphoid hyperplasia from malignancy and/or B-cell lymphoma from T-cell lymphoma, respectively. The BIOMED-2 PCR protocol has been successfully implicated in our laboratory to support the molecular diagnosis of lymphoid neoplasm. Our experience found an excellent sensitivity (95.7%) and specificity (100%) in the diagnosis of B-cell lymphoma. However, apparently lower clinical sensitivity (50%) was observed in the diagnosis of T-cell lymphoma using primer sets of TCRβ and TCRγ genes designed for western countries (91%). We hypothesized that T cell lymphoma might not be rearranged in our population. Alternatively, sequence variations (polymorphism) of the rearranged gene may compromise the annealing of PCR primers to DNA templates. To address this question, we established the 5’-rapid ampliphication of cDNA ends (5’-RACE), next generation sequencing (NGS), and algorithm for bioinformatic analysis of T-cell lymphoma. The aims of this pilot study are (1) to examine the distribution and frequency of TCRβ VDJ recombination in Taiwanese (2) to identify the impact of sequence variations (polymorphism) of TCRβ on primer annealing (3) to develop superior primer sets for Taiwanese in the diagnosis of T cell malignancy. With help of NGS and bioinformatics, this proposal is designed to develop a molecular diagnosis protocol for T cell lymphoma in Taiwan.
*關鍵字1 ： clonality
*關鍵字2 ： NGS
*關鍵字3 ： 5-RACE
*關鍵字4 ： T cell gene rearrangement
*關鍵字5 ： T cell lymphoma
* 服務機關：
* 第一作者： 陳怡伶
* 身分字號： *****63198
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 開啟檔案
審查委員意見： No experimental results are included. Acceptable if the text is revised accordingly.
審查委員意見： 建議可以口頭報告,作者用西方國家建議的BIOMED-2 protocol檢測台灣的淋巴瘤,發現B細胞淋巴瘤適用,但是T細胞淋巴瘤則不適用,因此,作者進一步用5'-RACE, NGS以及數據分析建立了屬
主任委員意見：
 
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