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歷 年 稿 件 內 容
 
*類別:
* 姓名: 陳綺鄉
投稿種類: 壁報
*中文投稿標題: 評估使用MALDI-TOF質譜儀快速診斷陽性血瓶菌種結果
*中文作者姓名列:
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*英文服務單位:
* 投稿摘要: Aim: We evaluated the reliability of the Bruker Daltoniks MALDI Biotyper system in species-level identification of bacteria directly from blood culture bottles. Material and Methods: An Saponin-lysing procedure was used to prepare a bacterial pellet from positive blood cultures for direct matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry analysis. A total of 399 blood culture positive samples were analyzed by MALDI Biotyper system (MBT, Bruker Daltonics, Germany) and compared with VITEK 2 (bioMerieux, Marcy lEtoile, France). Result: Identification results were concordant with those of the conventional culture-based method for 179(93.7%) gram-negatives and 81(64.8%) gram-positive species. Only 4 (21.1%) yeast species could be rapid identified by MALDI-TOF MS. Results were available in in less than 2 hours, suggesting that this approach is a reliable, time-saving tool for routine identification of bacteria species causing bloodstream infection. Conclusion: Identification was obtained for 93.7% of the pellets tested. Moreover, turn-around time is sharply reduced for communication of identification to the clinician. This fast and accurate method is promising.
*關鍵字1 : MALDI-TOF
*關鍵字2 : Bruker
*關鍵字3 : bioMérieux
*關鍵字4 : VITEK 2
*關鍵字5 : Blood culture
* 服務機關:
* 第一作者: 陳綺鄉
* 身分字號: *****05554
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審查委員意見: 建議刊登
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