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歷 年 稿 件 內 容
 
*類別：
* 姓名： 王秀梅
投稿種類： 壁報
*中文投稿標題： Aeromonas hydrophilia infection diagnosed by 16S rDNA sequencing – a case report
*中文作者姓名列：
*中文服務單位：
*英文投稿標題：
*英文作者姓名列：
*英文服務單位：
* 投稿摘要： Aeromonas and Vibrio are gram-negative, facultative anaerobic, and lactose-fermenting bacilli. They occasionally cause gastroenteritis and sepsis via water contamination and wound infection, respectively. Usually, Vibrio can grow in TCBS argar and in medium with NaCl, and is sensitive to O129. In contrast, Aeromonas usually cannot grow in these media and is resistant to O129. A 66-year-old male patient with a history of lung cancer and hypertension visited our hospital due to fever and severe cough. Blood culture proved pyemia. The results of traditional and commercial identification tests revealed Vibrio which could grow in medium with NaCl, expressed yellow colonies on TCBS agar, and was susceptible to O129. V. chleroae was suspected. However, the results of the commercial kit, VITEK 2 system (bioMerirux, Marcyl’Etoile, France), showed V. alginolyticus and V. cholerae. The specific species could not be effectively identified. Vibrio multiplex PCR analysis for V. cholerae, V. vulnificus, and V. alginolyticus also didn’t yield any expected products. Then, 16S rDNA sequencing revealed 99% genome similarity to Aeromonas hydrophilia. Finally, the result of PCR analysis with specific primers to Aeromonas hydrophilia matched with it. Sometimes, we cannot correctly identify the specific species of some microorganisms, because of unexpected biochemical reaction. As long as the molecular techniques are available, we can not only effectively resolve the dilemma that we encounter with traditional and commercial methods but speed clinical diagnosis and treatment.
*關鍵字1 ： Aeromonas hydrophilia
*關鍵字2 ： Vibrio
*關鍵字3 ： 16s rDNA
*關鍵字4 ： PCR
*關鍵字5 ： 0129
* 服務機關：
* 第一作者： 張嘉齡
* 身分字號： *****88014
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 開啟檔案
審查委員意見： 請改為方法建立，而非case report
審查委員意見： 建議刊登
主任委員意見：
 
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