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歷 年 稿 件 內 容
 
*類別：
* 姓名： 黃一聰
投稿種類： 壁報
*中文投稿標題： Application of PCR-RFLP for Identification and Genotype Characterization of Orientia tsutsugamushi Isolated from Humans
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*中文服務單位：
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*英文服務單位：
* 投稿摘要： Scrub typhus (ST), an acute febrile illness, is a notifiable infectious disease in Taiwan, and thousands of patients are suspected of ST infection annually. ST can be treated effectively with antibiotics and the fatality rate can reach to 50% in the absence of treatment. ST is caused by the obligate intracellular bacterium Orientia tsutsugamushi. To determine the serotype is difficult until the bacterium is cultured. Moreover, studies are time consuming and problematic for laboratory workers. In our study, O. tsutsugamushi was rapidly detected and typed by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis of the 56-kDa type-specific antigen (TSA) gene. We collected 3223 blood samples from eastern Taiwanese patients with suspected ST from 2002 to 2008. In total, 505 samples were found to be positive for ST infection by PCR. Four prototype and eleven Taiwanese genotypes were identified by RFLP analysis. Using the MEGA and PHYLIP software, we further analysed the genetic relatedness of the 56-kDa TSA gene of the twenty-one isolates used as well as of 104 DNA sequences previously deposited in GenBank. Fifteen RFLP genotypes cover all major phylogenetic branches, and each RFLP type reflects unique cluster. The PCR-RFLP is a sensitive, cheap, simple, rapid and reliable method for genotyping of O. tsutsugamushi. Our study demonstrated that the PCR-RFLP method may be used effectively to categorize clinical ST and be useful in public health investigations of O. tsutsugamushi.
*關鍵字1 ： Scrub typhus
*關鍵字2 ： Orientia tsutsugamushi
*關鍵字3 ： polymerase chain reaction (PCR)
*關鍵字4 ： restriction fragment length polymorphism (RFLP)
*關鍵字5 ： 56-kDa type-specific antigen (TSA)
* 服務機關：
* 第一作者： 黃一聰
* 身分字號： *****63275
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