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歷 年 稿 件 內 容
 
*類別: D組-分子醫學
* 姓名: 呂旭峰
投稿種類: 壁報
*中文投稿標題: α-水芹烯導致小鼠血癌細胞株(WEHI-3)凋零
*中文作者姓名列: 呂旭峰1、李慶孝2、鍾景光3
*中文服務單位: 振興醫療財團法人振興醫院1、仁德醫護管理專科學校2、中國醫藥大學生物技術學院3
*英文投稿標題: Alpha-Phellandrene-Induced Apoptosis in Mice Leukemia WEHI-3 Cells In Vitro
*英文作者姓名列: Lu Hsu-Feng1; Lee Ching Hsiao2; Chung Jing-Gung3
*英文服務單位: 1Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei;2Jen-The Junior College of Medicine, Nursing and Management, Miaoli County, Taiwan; 3Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.
* 投稿摘要: Although reports have shown that α-phellandrene (α-PA) is one of the monoterpenes and is often used in the food and perfume industry, our previous studies have indicated that α-PA promoted immune responses in normal mice in vivo. However, there is no available information to show that α-PA induced cell apoptosis in cancer cells, thus, we investigated the effects of α-PA on the cell morphology, viability, cell cycle distribution, and apoptosis in mice leukemia WEHI-3 cells in vitro. Results indicated that α-PA induced cell morphological changes and decreased viability, induced G0/G1 arrest and sub-G1 phase (apoptosis) in WEHI-3 cells. α-PA increased the productions of reactive oxygen species (ROS) and Ca+2 and decreased the levels of mitochondrial membrane potential (△Ψm) in dose- and time-dependent manners in WEHI-3 cells that were analyzed by flow cytometer. Results from confocal laser microscopic system examinations show that α-PA promoted the release of cytochrome c, AIF, and Endo G from mitochondria in WEHI-3 cells. These results are the first findings to provide new information for understanding the mechanisms by which α-PA induces cell cycle arrest and apoptosis in WEHI-3 cells in vitro.
*關鍵字1 : α-phellandrene
*關鍵字2 : monoterpenes
*關鍵字3 : apoptosis
*關鍵字4 : leukemia
*關鍵字5 : reactive oxygen species
* 服務機關:
* 第一作者: 呂旭峰
* 身分字號: *****35042
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審查委員意見: 建議入選
審查委員意見: OK
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