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歷 年 稿 件 內 容
 
*類別： D組-分子醫學
* 姓名： 呂旭峰
投稿種類： 壁報
*中文投稿標題： 薑黃素的代謝產物Bisdemethoxycurcumin會導致人類肺癌細胞株(NCI H460)細胞週期停滯於S期是透過內質網壓力的增加與粒線體膜電位的下降所促成
*中文作者姓名列： 呂旭峰1、李慶孝2、鍾景光3
*中文服務單位： 振興醫療財團法人振興醫院1、仁德醫護管理專科學校2、 中國醫藥大學生物技術學院3
*英文投稿標題： Bisdemethoxycurcumin-Induced S Phase Arrest through the Inhibition of Cyclin A and E and Induction of Apoptosis via Endoplasmic Reticulum Stress and Mitochondria-Dependent Pathways in Human Lung Cancer NCI H460 Cells
*英文作者姓名列： Lu Hsu-Feng1; Lee Ching Hsiao2; Chung Jing-Gung3
*英文服務單位： 1Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei;2Jen-The Junior College of Medicine, Nursing and Management, Miaoli County, Taiwan; 3Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.
* 投稿摘要： Curcuminoids are the major natural phenolic compounds found in the rhizome of many Curcuma species. Curcuminoids consist of a mixture of curcumin, demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC). Although numerous studies have shown that curcumin induced cell apoptosis in many human cancer cells, however, mechanisms of BDMC-inhibited cell growth and -induced apoptosis in human lung cancer cells still remain unclear. Herein, we investigated the effect of BDMC on the cell death via the cell cycle arrest and induction of apoptosis in NCI H460 human lung cancer cells. Flow cytometry assay was used to measure viable cells, cell cycle distribution, the productions of reactive oxygen species and Ca+2, mitochondrial membrane potential and caspase-3, -8 and -9 activity. DNA damage and condensation were assayed by Comet assay and DAPI staining, respectively. Western blotting was used to measure the changes of cell cycle and apoptosis associated protein expressions. Results indicated that BDMC significantly induced cell death through induced S phase arrest and induced apoptosis. Moreover, DMC induced DNA damage and condensation, increased ROS and Ca+2 productions and decreased the levels of △Ψm and promoted activities caspase-3, -8, and -9. Western blotting results showed that BDMC inhibited Cdc25A, cyclin A and E for causing S phase arrest, furthermore, promoted the expression of AIF, Endo G and PARP and the levels of Fas ligand (Fas L) and Fas were also upregulated. Results also indicated that BDMC increased ER stress associated protein expression such as GRP78, GADD153, IRE1α, IRE1β, ATF-6α, ATF-6β, and caspase-4. Taken together, we suggest that BDMC induced cell apoptosis through multiple signal pathways such as extrinsic, intrinsic and ES tress pathway.
*關鍵字1 ： Curcuminoids
*關鍵字2 ： demethoxycurcumin
*關鍵字3 ： bisdemethoxycurcumin
*關鍵字4 ： apoptosis
*關鍵字5 ： reactive oxygen species
* 服務機關：
* 第一作者： 呂旭峰
* 身分字號： *****35042
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審查委員意見： 建議入選
審查委員意見： OK
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