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歷年稿件內容

*類別：	B組-微生物、病毒
* 姓名：	李兆清
投稿種類：	壁報
*中文投稿標題：	北台灣某醫學中心運用新興技術 MALDI-TOF MS 系統鑑定厭氧菌
*中文作者姓名列：	李兆清1、余芳蘭1、楊婷雅1 、黃桂馨1、鄭佳玲1、黃綉茹1、洪經勝1、陳豐佳1、楊沂淵2、王炯中1
*中文服務單位：	臺北醫學大學市立萬芳醫院實驗診斷科1, 臺北醫學大學醫學科技學院醫學檢驗暨生物技術學系2
*英文投稿標題：	The Bruker Microflex Mass Spectrometry (MS), a Matrix-Assisted Laser Desorption Ionization Time-of-Flight MS System is an emerging technology for Identification of Anaerobic Bacteria : a surveillance in a medical center in northern Taiwan
*英文作者姓名列：	Lee Jau-Ching 1; Yu Fang-Lan 1; Yang Ting-Ya 1; Huang Guei-Sin 1; Cheng Chia-Ling 1; Huang Hsiu-Ru 1; Hung Ching-Sheng 1; Chen Feng-Chia 1; Yang Yi-Yuan 2; Wang Giueng-Chueng 1
*英文服務單位：	Department of Laboratory Medicine, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan1,School of Medical Laboratory Science and Biotechnology, College of Medical Science and Technology, Taipei Medical University, Taipei, Taiwan2
* 投稿摘要：	Background/Purpose: Conventional methods can identify anaerobic bacteria; but they are troublesome, time-consuming and requires more expertise. Many commercial biochemical systems are available for identification of anaerobes. But these assays are technically complex, cumbersome and expensive. Hence, alternative techniques with improved sensitivity, specificity, cost-down and time-effectiveness are required to facilitate early treatment. The purpose of this retrospective study is to investigate the performance of the Bruker Microflex MS (B-M MS) in the identification of anaerobes. Methods: Consecutive, a broad diversity of anaerobes were tested during a period of three months (August 2015 to October 2015). MALDI-TOF analysis was performed using a B-M MS. Captured spectra were analysed using a MALDI-TOF Biotyper automated control and the Bruker Biotyper 3.1 software and library (Bruker Daltonics). Results were interpreted as follows: < 1.7 as unreliable identification, 1.7-1.999 as valid identification to the genus level, and ≥ 2.0 as definite identification of the genus and probable identification of the species. Results: A total of 215 anaerobes comprising 78 GNB, 15 GNC, 41 GPC, and 81 GPB made up of 13 genera and 36 species were tested. The B-M MS correctly identified 158 (73.5%) isolates to the species level and an additional 57 (26.5%) at the genus level. In particular, the B-M MS correctly identified clinically relevant and frequently isolated anaerobes to the species level. Conclusions: The B-M MS showed reliable identifications for clinically relevant anaerobes.
*關鍵字1 ：	MALDI-TOF MS
*關鍵字2 ：	Bruker Microflex MS (B-M MS)
*關鍵字3 ：	Anaerobic bacteria
*關鍵字4 ：	anaerobe
*關鍵字5 ：	無
* 服務機關：
* 第一作者：	李兆清
* 身分字號：	*****65135
其他投稿作者：	余芳蘭
身分字號：	*****32399
其他投稿作者：	楊婷雅
身分字號：	*****91321
其他投稿作者：	黃桂馨
身分字號：	*****04159
其他投稿作者：	鄭佳玲
身分字號：	*****85876
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上傳稿件檔：
審查委員意見：	單一醫院利用MALDI TOF的厭氧結果分析
審查委員意見：	不OK
主任委員意見：