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歷 年 稿 件 內 容
 
*類別： F組-其他
* 姓名： 張凱復
投稿種類： 壁報
*中文投稿標題： 探討天然植物JsCsPe萃出物對抗惡性腦腫瘤於體內及體外之抑癌作用機制
*中文作者姓名列： 張凱復1, #,李健如2, 洪芃昀2, 周恬而2, 李名世2,3, 蔡菁華1, 蔡女滿2,3 *
*中文服務單位： 中山醫學大學醫研所1,中山醫學大學醫學檢驗暨生物技術學系所2,中山醫學大學附設醫院檢驗科3
*英文投稿標題： To investigate the anti-tumor effects and mechanisms of JsCsPe extract on GBM tumor in vitro and in vivo
*英文作者姓名列： Kai-Fu Chang 1,#, Chien-Ju Lee 2, Peng-Yun Hong 2, Tien-Erh Chou 2, Ming-Shih Lee2,3, Jinghua Tsai Chang 1, Nu-Man Tsai2,3, *
*英文服務單位： 1Institute of Medicine of Chung Shan Medical University; 2School of Medical Laboratory and Biotechnology, Chung Shan Medical University; 3Clinical Laboratory, Chung Shan Medical University Hospital, Taichung, Taiwan.
* 投稿摘要： Glioblastoma multiforme (GBM) has poor prognosis and the five-year survival rate is less than 5%. Because of blood-brain barrier, the chemodrugs usually have low responses for GBM therapy in clinical. Recent research reports show that GBM have been progressively resistant to clinical drugs. Thus, to develop a more effective drug for GBM therapy is urgent requirement. JsCsPe, a natural plant, is used in traditional medicine for anti-bacteria growth, promoting liver detoxification and improvement of gout or rheumatism. Some studies show that water extract of JsCsPe could inhibit tumor cell growth and induce cell apoptosis. Nevertheless, the detail mechanisms of against GBM tumor are still unknown. In previous study, we found out that JsCsPe extract had more anti-tumor effects than its water extract through a series screening of bioactivity selective conditions. The aim of this research is to examine the anti-tumor effects and mechanisms of JsCsPe extract on GBM tumor in vitro and in vivo. The results showed JsCsPe extract had more cytotocixity than clinical drug Temozolomide (TZM) on GBM cells. JsCsPe extract had synergistic effect which combined with TZM. However, JsCsPe extract had lower cytotocixity on normal cells. After JsCsPe extract treatment that induced cell cycle arrest at G0/G1 phase and increased population of Sub G1 phase. Besides, GBM cells which treated JsCsPe extract showed TUNEL positive and formed DNA fragmentation, chromatin condensation, and apoptotic bodies. Using JsCsPe extract treatment, the cycle regulators (P21, P53 and PP53) were up-regulation, and cell cycle related proteins (Cyclin A, Cyclin B1, Cyclin D1, CDK2, CDK4) were down-regulation. In addition, JsCsPe extract treated cells showed apoptosis associated moleculars of extrinsic pathway (FAS, FASL, and cleaved caspase-8), intrinsic pathway (AIF, Bax, and cleaved caspase-9) and cleaved caspase-3 were increased. On the other hand, when GBM cells pretreated caspased-3 inhibitor, the JsCsPe extract inducing caspase-3 activation pathway was blocked that indicating it could induce caspase cascade activation to trigger cell apoptosis. The results of in vivo study demonstrated that JsCsPe extract could suppress GBM tumor growth, prolong animal survival time and had low or no physiological and pathological toxicity by detection of serum biochemistry and organ damage. Utilizing immunochemistry analysis, the expression of proliferation marker (PCNA) was decreased, but the apoptosis maker (cleaved caspased-3) was increased after JsCsPe extract treatment. In conclusion, JsCsPe extract could induce cell cycle arrest and GBM cell apoptosis which go through caspase cascade activation apoptosis pathway, prolong animal lifespan. Therefore, this evidence offered a preclinical validation and mechanism definition of a new effective drug, JsCsPe extract, on GBM therapy in the future.
*關鍵字1 ： Glioblastoma multiforme (GBM)
*關鍵字2 ： Traditional medicine
*關鍵字3 ： Apoptosis
*關鍵字4 ： Anti-tumor
*關鍵字5 ： Chemotherapy
* 服務機關：
* 第一作者： 張凱復
* 身分字號： *****36554
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