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歷年稿件內容

*類別：	F組-其他
* 姓名：	羅彗菱
投稿種類：	壁報
*中文投稿標題：	研究植物PuJiCu粗萃物對口腔癌細胞之抑癌作用機轉
*中文作者姓名列：	羅彗菱1,#, 廖虹琇2, 陳柏村1,謝明昌1,2,王政光3,王啟屏1,2,李名世1,2,蔡女滿1,2,*
*中文服務單位：	中山醫學大學附設醫院檢驗科1,中山醫學大學醫學檢驗暨生物技術學系所2, 仁德醫護管理專科學校醫事檢驗科3,
*英文投稿標題：	To study the anti-cancer effects and mechanisms of PuJiCu extract in oral cancer
*英文作者姓名列：	Hui-Ling Lo 1,#, Hui-Ling Lo 2, Po-Tsun Chen 1, Ming-Chang Hsieh 1,2, Cheng-Kuang Wang 3, Chi-Pin Wang 1,2, Ming-Shih Lee 1,2, Nu-Man Tsai 1, 2, *
*英文服務單位：	1Clinical Laboratory, Chung Shan Medical University Hospital, 2School of Medical Laboratory and Biotechnology, Chung Shan Medical University, 3Department of Medical Technology, Jen-The Junior College of Medicine, Nursing and Management
* 投稿摘要：	Oral cancer is one of the ten most common cancers in the world. Furthermore, the incidence of oral cancer is higher in Taiwan due to the habits of tobacco, alcohol and areca use. The 5-year survival rate of oral cancer is 63%, but it declined to 37% with distant metastasis stage. Besides, about 18% of patients have distant metastasis at the time of diagnosis. Therefore, it is urgent required to development a new anti-cancer agent. In traditional medicine, PuJiCu can be used on anti-acne, aromatherapy, bladder infection, cellulite, and detoxification and so on. Thus, the purpose of this project is to investigate the anti-cancer effects and mechanisms of PuJiCu extract in oral cancer. In previous screening experiments, the results showed that both PuJiCu extract and 5-FU could inhibit oral cancer cells growth in a dose and time dependent manner, however, the cytotoxicity of 5-FU is higher than PuJiCu extract treatment in normal cells. Additionally, PuJiCu extract combined with 5-FU had synergistic effect, with the combination index (CI) <1, and the cytotoxicity of combinatorial treatment was greater than 5-FU along. Next, we focused on the anti-cancer effects of PuJiCu extract in vitro. At first, treatment with PuJiCu extract, the cell cycle distribution was analyzed by flow cytometry and these results showed that PuJiCu extract induced G0/G1 phase arrest and increased sub-G1 phase cell population. To determine whether PuJiCu extract induced cell apoptosis in oral cancer cells, TUNEL assay was performing to observe cell death morphology. The results demonstrated that oral cancer cells were formed apoptosis morphology including apoptotic bodies, DNA fragmentation, DNA condensation and anoikis after PuJiCu extract treatment indicated that PuJiCu extract could induce cell apoptosis. PuJiCu extract treatment also led to altered the expression of several cell cycle related proteins (p-Rb, Rb, Cdk2, Cdk4, CyclinA, CyclinB1, CyclinD1) and cell cycle regulators (p21, p53 and p-p53). To gain insight into the mechanisms of apoptosis, the results indicated that both extrinsic pathway (Fas, FasL and caspase 8) and intrinsic pathway (Bax and caspase 9) were activated, and the expression of apoptosis related protein (pro-caspase 3, 8 and 9) were reduced after PuJiCu extract treatment. Furthermore, PuJiCu extract treatment could reduce the expression of angiogenesis and metastasis related protein in oral cancer cells. In conclusion, PuJiCu extract could induce cell cycle arrest at G0/G1 phase and through extrinsic and intrinsic pathway to trigger cell apoptosis. The combination of PuJiCu extract with 5-FU also had synergistic effects in oral cancer cells. Besides, PuJiCu extract could reduce angiogenesis and metastasis related proteins expression in oral cancer cells. Therefore, PuJiCu extract may be a potential chemotherapy agent in oral cancer therapy.
*關鍵字1 ：	Oral cancer
*關鍵字2 ：	cell cycle
*關鍵字3 ：	combination index
*關鍵字4 ：	apoptosis
*關鍵字5 ：	anti-cancer agent
* 服務機關：
* 第一作者：	羅彗菱
* 身分字號：	*****48360
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