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歷 年 稿 件 內 容
 
*類別： B組-微生物、病毒
* 姓名： 黃玉雯
投稿種類： 壁報
*中文投稿標題： 多重抗藥性金黃色葡萄球菌攝取丙胺酸對其表面電荷、乳酸鏈球菌素及抗菌劑敏感性影響之探討
*中文作者姓名列： 黃玉雯1,2、李尹平3、李元明1,4、陳莉臻3、林世斌2,3
*中文服務單位： 宜蘭國立陽明大學醫院檢驗醫學科1,國立宜蘭大學生物資源學院碩士在職專班2,國立宜蘭大學食品科學系3,生物資源學院宜蘭縣醫事檢驗師公會 4,台灣
*英文投稿標題： The influence of alanine uptake on methicillin-resistant Staphylococcus aureus for its cell surface charges and sensitivity to nisin and antibacterial agents
*英文作者姓名列： Yu-Wen Huang 1,2, ; Yin-Pin Lee3 ; Yuan-Ming Lee 1,4 ; Li-Chen Chen3 ; Shih-Bin Lin2,3
*英文服務單位： Department of Laboratory Medicine, National Yang-Ming University Hospital, I-Lan County1, Department of Biotechnology and Animal Science , EMA program in College of Bioresources, National Ilan University, I-Lan County2, Department of Food Science, National Ilan University, I-Lan County3, Yilan Association of Medical Technologists, I-Lan County4, Taiwan
* 投稿摘要： The drug sensitivity of methicillin-resistant S. aureus (MRSA) to current antibacterial agents was found decreasing in these years. Looking for an alternative antibacterial agent has become urgent. The goal of this study is to analyze the antibacterial effects of nisin against various S. aureus strains, and, at the same time, to evaluate the effect of alanine uptake on the change in cell surface charges, following which their drug sensitivity may increase. Results demonstrated that the minimum inhibitory concentrations (MICs) of vancomycin for these MRSA strains were ranged between 0.5~1.0μg mL-1, and the MICs of nisin for these MRSA strains were ranged between 1.25 – 20 IU mL-1 with an average of 10 IU mL-1. Similar MIC range, 5-20 IU mL-1 , with an average of 10 IU mL-1, was obtained for MSSA stains. An increased sensitivity of most of the MRSA (77%) to nisin was found, with average MICnisin reduced to 49 %, after being cultured with L-alanine supplement (1%, w/v); it seemed that MRSA with MICVa of 1.0μg mL-1 was affected most significant. The cytochrome c (cyt C) unbound test results demonstrated the L-alanine uptake could decrease the bounding capacity of cytC to strains, which indicated that the negative charge on cell surface was decrease. Furthermore, the study found that the MRSA strain, with MICVa of 1.0μg mL-1, with less negative charge on cell surface was more vulnerable to nisin. These finding will be help to establish the studies on MRSA for an alternative antibacterial agents.
*關鍵字1 ： methicillin-resistant S. aureus
*關鍵字2 ： nisin
*關鍵字3 ： alanine
*關鍵字4 ： vancomycin
*關鍵字5 ： cytochrome c unbound test
* 服務機關：
* 第一作者： 黃玉雯
* 身分字號： *****58787
其他投稿作者： 李尹平
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其他投稿作者： 李元明
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其他投稿作者： 陳莉臻
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上傳稿件檔：
審查委員意見： 測試菌株數量不明 加ala對nicin 或Van 影響定義不清
審查委員意見： 不OK
主任委員意見：
 
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