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歷 年 稿 件 內 容
 
*類別： B組-微生物、病毒
* 姓名： 田霓
投稿種類： 壁報
*中文投稿標題： 前處理透析液檢体可提升微生物培養的檢出率及有效縮短培養時間
*中文作者姓名列： 田霓1,2、林秀姍1、張志忠3,4
*中文服務單位： 中國醫藥大學附設醫院檢驗醫學部1、中國醫藥大學醫學檢驗暨生物技術學系2、中國醫藥大學附設醫院內科部腎臟科3、中國醫藥大學醫學系4
*英文投稿標題： Pretreatment of dialysate with bacterial washing and concentrating can reduce the bacterial culture report time and enhance the positive culture report rate.
*英文作者姓名列： Ni Tien1,2;Hsiu-Hsien Lin1;Chiz -Tzung Chang3,4
*英文服務單位： Department of Laboratory Medicine, China Medical University Hospital, Taiwan.1; Department of Medical Laboratory Science and Biotechnology, China Medical University,Taiwan 2; Division of Nephrology, China Medical University Hospital, Taiwan 3, College of Medicine, China Medical University, Taiwan 4
* 投稿摘要： Bacteria infection in peritoneal dialysis (PD)-related peritonitis is the most common cause. PD peritonitis is diagnosed by dialysate culture and it takes about 3-7 days. A fast and accurate method is therefore mandatory. PD patients with abdominal pain and turbid dialysate were included in this study. In conventional culture method, 10 ml dialysate were down by automatic blood culture system. The positive bacteria was sub-cultured. Microbial identification was done by MALDI-TOF and antibiotic susceptibility test was performed. We accelerated bacteria growth by concentration and washing (C&W) methods. We were using the concentrated and repeated saline wash before bacterial culture. We compared the results of two methods of culture results differences, and the length of time. There were 79 PD peritonitis patients, including the 16 culture were blind control tests. The blind tests results of two methods were identical. The consistency between these 2 methods was 55/79 (70%). There were 24 conventional reports different from the paired reports by C&W method. There were 3 C&W reports were negative and 10 negative were reported by conventional method, but 11 poly-species were cultured by C&W methods. The culture positive rate was lower by conventional (45/79;57%) than by C&W (72/79;91%)(p<0.001). The median culture report time by conventional method was 91 hours but 37 hours by C&W method (p<0.001). Dialysate culture by sample pretreatment with bacterial washing and concentration can reduce bacterial culture time and enhance positive bacterial culture rates.
*關鍵字1 ： peritoneal dialysis (PD)-related peritonitis
*關鍵字2 ： conventional culture
*關鍵字3 ： concentration and washing (C&W) methods
*關鍵字4 ： 無
*關鍵字5 ： 無
* 服務機關：
* 第一作者： 田霓
* 身分字號： *****92636
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