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歷 年 稿 件 內 容
 
*類別： D組-分子醫學
* 姓名： 呂汶紋
投稿種類： 壁報
*中文投稿標題： 研究經藥物篩選所得到之艾達黴素的分子作用機制:增強干擾素作用以抑制腸病毒71型複製
*中文作者姓名列： 呂汶紋1,趙偉勝1, 吳佩蓉2,龔思豪2
*中文服務單位： 振興醫療財團法人振興醫院臨床病理科1,國立陽明大學醫學生物技術暨檢驗學系2
*英文投稿標題： Action Mechanisms of Idarubicin Identified from A Drug Screen:Enhancement of Interferon Effect against Enterovirus 71 Replication
*英文作者姓名列： Lu Wen-Wen1, Chao Wei-Sheng1,Wu Pei-Jung2, Kung Szu-Hao2
*英文服務單位： Department of Clinical Pathology, Cheng Hsin General Hospital, Taipei, Taiwan1; Department of Biotechnology and Laboratory Science in Medicine, National Yang-Ming University, Taipei, Taiwan2
* 投稿摘要： Backgrounds: EV71 infections could cause neurological diseases in young children. Type I interferon (IFN) signaling is important for innate antiviral responses. However, EV71 have evolved measures to counteract the IFN responses. Our showed Idarubicin (IDR) displays anti-EV71 activities in sub-cytotoxic concentrations. Moreover, IDR has recently been reported to increase IFN stimulating response element (ISRE) activity. However, the molecular mechanism of IDR underlying the IFN signal induction and antiviral effects remains to be explored. Materials and Methods: Western blot was used to probe the protein level of components in the IFN-activating pathway. The ISRE activity was measured by the dual luciferase assay. Confocal analysis was used to probe the localization of the interferon regulatory factor 9(IRF9). IFA and cytotoxicity assay were used to measure the combined effects of IDR and IFN-β. Results: We showed IDR promoting IFN signaling during EV71 infection. IDR raised the protein level of IRF9 but not in other components in the JAK-STAT pathway. IDR treatment resulted in IRF9 translocation from the cytoplasm to the nucleus. Finally, synergistic effect against EV71 infection was observed yet it also lowered down the cell viability. We have attempted to elaborate the molecular mechanisms underlying the type I IFN-activating pathway induced by IDR in the context of EV71 infection. This finding could lead to develop a broad-spectrum antiviral drug based on IFN signal stimulation.
*關鍵字1 ： Enterovirus 71
*關鍵字2 ： Idarubicin
*關鍵字3 ： Interferon
*關鍵字4 ： IFN stimulating response element
*關鍵字5 ： ISRE
* 服務機關：
* 第一作者： 呂汶紋
* 身分字號： *****58949
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