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歷 年 稿 件 內 容
 
*類別： D組-分子醫學
* 姓名： 簡明志
投稿種類： 壁報
*中文投稿標題： 評估使用Luminex MultiCode-RTx Kit檢測血液中EB病毒
*中文作者姓名列： 簡明志1*，彭成立1，王怡惠1、鍾欣怡1、李筱薇1、廖淑容1、李詩儀1、闕宗熙1
*中文服務單位： 三軍總醫院臨床病理科
*英文投稿標題： Evaluation and routine use of the Luminex MultiCode-RTx EBV virus for blood in a local hospital.
*英文作者姓名列： Ming Jr Jian1*, Cherng-Lih Perng1, Yi-Hui Wang1, Xin-Yi Zhong1, Xiao-Wei Li1, Shu-Rung Liao1, Shi-Yi Li1, Tzong-Shi Chiueh1
*英文服務單位： 1Division of Clinical Pathology, Tri-Service General Hospital, NDMC
* 投稿摘要： Background: Epstein-Barr virus (EBV) is a widely disseminated herpesvirus, which is spread by intimate contact between susceptible persons and asymptomatic EBV shedders. The majority of primary EBV infections throughout the world are subclinical and unapparent. Epstein-Barr virus (EBV) is a double-stranded DNA virus which exists in either a lytic or latent phase of infection. Polymerase chain reaction (PCR) has been pioneered as a technology to rapidly and accurately detect and quantify EBV DNA from clinical specimens. Epstein-Barr virus (EBV) has been implicated in the pathogenesis of several malignancies including non-Hodgkin and Hodgkin lymphomas and nasopharyngeal carcinoma .The quantification of Epstein-Barr virus (EBV) DNA from the peripheral blood is often used to evaluate patients suspected of having disseminated EBV infection. MultiCode products from Luminex offer a flexible platform for both real-time and multiplex PCR-based assays. MultiCode products are based upon the unique patented MultiCode bases,isoC and isoG. This property enables site-specific incorporation of the isobases during amplification.The Luminex MultiCode-RTx EBV PCR kit was FDA-cleared for detection of EBV infection. Objectives: Here we describe the verification and our experience at our hospital with the off-label use of MultiCode-RTx kit to detect EBV in blood. Methods: Verification was performed using clinical specimens and proficiency testing panels. Laboratory-developed, real-time EBV PCR (Ref: Lin JC. et al. N Engl J Med. 2004;350:2461-70.) was the major reference method. Results: The MultiCode-RTx EBV kit demonstrated limit of detection (LOD) of 1-10 copies per reaction in serum. Analytical accuracy and precision were excellent, and specificity was 100% compared with our reference method. Sensitivity was 95%, one specimens missed by MultiCode-RTx in 50 blood specimens . The Luminex MultiCode-RTx-EBV PCR kit could serve as an useful alternative to our laboratory-developed real-time EBV PCR for detection of EBV in blood specimens received by diagnostic laboratories.
*關鍵字1 ： EBV
*關鍵字2 ： MultiCode
*關鍵字3 ： Real time PCR
*關鍵字4 ： Diagnosis
*關鍵字5 ： Luminex
* 服務機關：
* 第一作者： 簡明志
* 身分字號： *****00192
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