2024-01-10　『2024第15屆亞太醫學檢驗科學國際研討會』開放報名至3月15日止，請會員把握機會踴躍參與！　 2023-07-01　會員如有權益相關問題及對本會有任何建議或意見回饋，歡迎利用電子信箱或致電本會，謝謝您!! 　 2015-02-02　醫事檢驗師宣誓誓詞，誓詞內容請至文件下載區查閱，並請協助推廣，謝謝！　

:::

歷年稿件內容

*類別：	D組-分子醫學
* 姓名：	劉原智
投稿種類：	壁報
*中文投稿標題：	可定量檢測NS5A突變之焦磷酸定序方法開發
*中文作者姓名列：	林彥廷 1，徐武琦 1，許琳岡 1，劉原智 1，2，葉育銘 1
*中文服務單位：	1，普生股份有限公司 2，居禮股份有限公司
*英文投稿標題：	Development of the de novo Pyrosequencing assay for Quantitative Analysis of HCV NS5A Mutation
*英文作者姓名列：	Yen-Ting Lin 1, Wu-Chi Hsu 1 , Lin-Kang Hsu 1, Yua-Zhi Liu 1,2, Yu-Ming Yeh 1
*英文服務單位：	1, General Biologicals Corporation, 2, Curiemed Corporation
* 投稿摘要：	Oral therapeutic agents that target HCV NS5 replication complex, for example Daclatasvir, has been known to provide high sustained virological response (SVR) in patients with HCV 1b genotype. However, patients harbored NS5A L31M/V or Y93H mutation has been shown getting no benefit from taking the oral therapeutic agents. Analyzing the status of HCV NS5A mutation before taking therapeutic agents, therefore, is urgent and essential. Here, we present a sensitive and quantitative assay, with the principle of pyrosequencing, to detect the viral strains with HCV NS5A mutations. We utilized a specific biotinylated-PCR primers to target NS5A gene and amplified the fragments with one-step RT-PCR. The de novo pyrosequencing assay was performed with QIAGEN PyroMark Q24 system. The operation time of the assay is around 6 hours with 4 hours hand-off, demonstrating the operational efficiency. By analyzing the codons of target sites, not only the presence of L31M/V and Y93H could be distinguished but the quantified information of mutant strains could be provided. The analytic sensitivity of the assay was evaluated with mixtures of wild type and mutant specimens. And the results demonstrated that approximately 5% of mutant could be detected directly, which is way more sensitive than traditional Sanger sequencing. The ability to obtain quantitative results would be very helpful for physicians to evaluate the therapeutic efficacy of HCV oral therapeutic agents.
*關鍵字1 ：	HCV
*關鍵字2 ：	NS5A mutation
*關鍵字3 ：	Pyrosequencing
*關鍵字4 ：	Quantitative assay
*關鍵字5 ：	NA
* 服務機關：
* 第一作者：	林彥廷
* 身分字號：	*****47295
其他投稿作者：
身分字號：
其他投稿作者：
身分字號：
其他投稿作者：
身分字號：
其他投稿作者：
身分字號：
其他投稿作者：
身分字號：
其他投稿作者：
身分字號：
其他投稿作者：
身分字號：
上傳稿件檔：
審查委員意見：	OK
審查委員意見：	無意見
主任委員意見：