2024-01-10　『2024第15屆亞太醫學檢驗科學國際研討會』開放報名至3月15日止，請會員把握機會踴躍參與！ 　 2023-07-01　會員如有權益相關問題及對本會有任何建議或意見回饋，歡迎利用電子信箱或致電本會，謝謝您!! 　 2015-02-02　醫事檢驗師宣誓誓詞，誓詞內容請至文件下載區查閱，並請協助推廣，謝謝！ 　

:::

歷 年 稿 件 內 容
 
*類別： D組-分子醫學
* 姓名： 劉原智
投稿種類： 壁報
*中文投稿標題： B型肝炎表面抗原缺失突變檢測--一種高解析度的診斷方法
*中文作者姓名列： 徐武琦 1，劉原智 1，2，葉育銘 1
*中文服務單位： 1，普生股份有限公司 2，居禮股份有限公司
*英文投稿標題： HBV Pre-S deletion mutation analysis—a high resolution diagnosis method
*英文作者姓名列： Wu-Chi Hsu 1, Yuan-Zhi Liu 1, 2, Yu-Ming Yeh 1
*英文服務單位： 1, General Biologicals Corporation, 2, Curiemed Corporation
* 投稿摘要： The Pre-S region(nucleotides 2854-155) locates at the S gene(encodeing the surface protein) of HBV DNA genome and consists of the Pre-S1 and Pre-S2 domains. A meta-analysis with more than 10,000 cases and dozens reports all indicate that infection of HBV with Pre-S mutant is associated with 3.77-fold increased risk of HCC. To evaluate the performance of the Pre-S deletion mutation detection system, several HBV infected serum samples with different viral titer were collected. And viral DNA was extracted with 300 uL serum. A set of Pre-S primers, which is applicable for all HBV genotypes, was used to amplify the target region with highly fidelity PCR polymerase. Results with high resolution came out via a technology called capillary electrophoresis (CE). This analytic method was accomplished by Qsep100TM machine from BiOptic Inc. This Pre-S PCR system has been successfully verified with many clinical samples, and it could be applied to samples with different HBV genotypes. Traditional method often use agarose gel system to bring out results. However, it may be not applicable when it comes to samples with low viral titer. Now, this problem can be solved with the capillary electrophoresis platform. Capillary electrophoresis not only can detect the pre-S signal at E+03 viral titer grade but also provide a system with analyzing consistency. To sum up, the capillary electrophoresis platform is a high resolution and sensitive method for detection of Pre-S deletion, especially for low viral titer samples.
*關鍵字1 ： HBV
*關鍵字2 ： Pre-S deletion
*關鍵字3 ： Molecular diagnosis
*關鍵字4 ： Capillary electrophoresis
*關鍵字5 ： NA
* 服務機關：
* 第一作者： 徐武琦
* 身分字號： *****17204
其他投稿作者：
身分字號：  
其他投稿作者：
身分字號：  
其他投稿作者：
身分字號：  
其他投稿作者：
身分字號：  
其他投稿作者：
身分字號：  
其他投稿作者：
身分字號：  
其他投稿作者：
身分字號：  
上傳稿件檔：
審查委員意見： 不OK
審查委員意見： 缺乏具體的數據呈現研究成果
主任委員意見：
 
回上頁