2025-07-15　恭賀 張璧月醫檢師 當選『第16屆桃園市醫事檢驗師公會理事長』!!! 　 2025-06-30　恭賀 邱仁祥醫檢師 當選『第17屆南投縣醫檢師公會理事長』!!! 　 2023-07-01　會員如有權益相關問題及對本會有任何建議或意見回饋，歡迎利用電子信箱或致電本會，謝謝您!! 　 2015-02-02　醫事檢驗師宣誓誓詞，誓詞內容請至文件下載區查閱，並請協助推廣，謝謝！ 　

:::

歷 年 稿 件 內 容
 
*類別：
* 姓名： 李宜蓁
投稿種類： 壁報
*中文投稿標題： Correlation of Human follicular fluid Hedgehog contents with oocyte quality
*中文作者姓名列：
*中文服務單位：
*英文投稿標題：
*英文作者姓名列：
*英文服務單位：
* 投稿摘要： Human follicular growth directly affects oocyte quality and ovarian steroidogenesis. Follicular growth involves granulosa-thecal interactions encompassing endocrine, paracrine, and autocrine regulatory mechanisms, through which follicular size, follicular fluid volume and contents, and oocyte quality are determined. Eventually, fertilization rate, early embryogenesis, and the success rates of IVF-ET treatment cycles are affected. Hedgehog is a major regulatory gene during embryonic development. Previous reports indicated that Hedgehog and its downstream factors are expressed in murine granulosa cells and corpus lutea. Supplementation of Hedgehog protein in culture system can promote follicular growth and granulosa cell proliferation. Furthermore, it was shown that Hedgehog protein was secreted in the granulosa cell to promote thecal cell activities. Additionally, we have found in our previous studies that Hedgehog protein is enriched in porcine follicular fluid and oocyte maturation is promoted with Hedgehog supplementation under in vitro culture conditions. In contrast, Hedgehog signaling blockage by cyclopamine (a Hedgehog inhibitor) leads to insufficient oocyte maturation, suggesting that Hedgehog contents in follicular fluid may affect oocyte quality. However, whether Hedgehog protein is enriched in human follicular fluid and how does it affect human oocyte quality remain not yet reported. In this study, we investigated 119 oocytes undergoing IVF-ET treatment cycles from women with infertility. Follicular fluid Hedgehog protein contents were measured by using enzyme-linked immunological assay (ELISA) analysis and the data were correlated with follicular diameter, oocyte maturity, fertilization rate, pre-implantation embryogenesis, and implantation rate. The results showed that Hedgehog contents in the follicular fluid positively affect oocyte maturation and subsequent success rates of IVF-ET. The present results may be used for assessment of oocyte quality and may provide the baseline information for promoting IVF-ET success rate through Hedgehog supplementation.
*關鍵字1 ： Hedgehog protein
*關鍵字2 ： oocyte
*關鍵字3 ： IVF-ET
*關鍵字4 ： follicular fluid
*關鍵字5 ： follicular growth
* 服務機關：
* 第一作者： 李宜蓁
* 身分字號： *****34960
其他投稿作者：
身分字號：  
其他投稿作者：
身分字號：  
其他投稿作者：
身分字號：  
其他投稿作者：
身分字號：  
其他投稿作者：
身分字號：  
其他投稿作者：
身分字號：  
其他投稿作者：
身分字號：  
上傳稿件檔：
 開啟檔案
審查委員意見： 無意見
審查委員意見： ok
主任委員意見：
 
回上頁