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歷 年 稿 件 內 容
 
*類別:
* 姓名: 賴志銘
投稿種類: 壁報
*中文投稿標題: Fibronectin degradation by matrix metalloproteinase-2 and -9
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*中文服務單位:
*英文投稿標題:
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* 投稿摘要: Toxoplasma gondii is a zoonotic parasite and its infection in human can induce toxoplasmic encephalitis. In this study, astroglia were infected with the TS-4 strain T. gondii tachyzoite in vitro to investigate the changes of matrix metalloproteinase (MMP)-2, MMP-9 and their substrate fibronectin. MMP-2 and MMP-9 were significantly increased at 1 h, 6 h and 12 h post-infection (PI) in the cell homogenates, and increased at 6 h, 12 h, 24 h and 48 h PI in the cultured supernatants. Fibronectin degradation also occurred at the same time points. In addition, immunocytochemistry showed that MMP-2 and MMP-9 localized in the cytoplasm, and MMP-2 and MMP-9 interacted with fibronectin, respectively. These results suggest that MMP-2 and MMP-9 induction from astroglia may contribute to extracellular matrix degradation in toxoplasmosis. Thus, we hypothesize that MMP-2 and MMP-9 cleave fibronectin and may contribute to the astroglia reaction and leukocyte migration to the sites of T. gondii replication during toxoplasmic encephalitis.
*關鍵字1 : astroglia
*關鍵字2 : fibronectin
*關鍵字3 : MMP-2
*關鍵字4 : MMP-9
*關鍵字5 : Toxoplasma gondii
* 服務機關:
* 第一作者: 賴志銘
* 身分字號: *****91321
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