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歷 年 稿 件 內 容
 
*類別：
* 姓名： 辜明慧
投稿種類： 壁報
*中文投稿標題： 透過蛋白質體學之比較分析搜尋與神經母細胞瘤缺氧反應及癌症轉移之生物標記 Biomarker Discovery of Hypoxia and Metastasis-related Proteins i
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* 投稿摘要： Neuroblastoma (NB) is the most common malignant solid tumor with unfortunate outcomes in many infants and children. This tumor arises from the sympathetic nervous system. The primary non-metastatic NB initiates at a localized area and is often resectable with good prognosis, but once metastasized, it is unresectable and resistant to chemotherapy while restraining tumor spread. The major type of NBs is the aggressive metastatic tumor. Furthermore, solid tumor cells have to overcome hypoxia and deficiency of nutrient. To ensure a sufficient supply of oxygen and nutrients, tumor metastasis or angiogenesis is probably a strategy for tumor growing in hypoxia. Therefore, the searching of differentially expressed hypoxia-induced and metastasis-related proteins, which are the aims of my thesis works, are important for the development of therapeutic target or diagnostic biomarker. The first project applied SILAC–based quantitative proteomics approach analyze hypoxia-induced protein in the secretome and cell lysate proteomes of NB cell lines, SH-SY5Y and SK-N-BE(2), cultured in hypoxia or normoxia condition. A total of 228 (6,862 peptides) and 286(9,601 peptides) SILAC-labeled proteins were identified by LC-MS/MS in the secretomes of SH-SY5Y and SK-N-BE(2) cells, respectively, and 338(9,154 peptides)and 374(9,603 peptides) SILAC-labeled proteins in the cell lysates of SH-SY5Y and SK-N-BE(2) cells, respectively. These proteins were further analyzed with the public NV microarray data, in-house consolidated secretome databases, and membrane protein database to narrow down the hypoxia related candidates. In my study, a number of novel hypoxia-related candidates including FN1 (fibronectin 1) and MMP2 (matrix metalloproteinase 2) were identified.
*關鍵字1 ： Comparative proteomics analysis
*關鍵字2 ： Hypoxia effect
*關鍵字3 ： Biomarker discovery
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* 第一作者： 辜明慧
* 身分字號： *****21584
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