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歷 年 稿 件 內 容
 
*類別：
* 姓名： 楊雅倩
投稿種類： 口頭
*中文投稿標題： Shisa3基因高度甲基化為大腸直腸癌新預後標記
*中文作者姓名列：
*中文服務單位：
*英文投稿標題：
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*英文服務單位：
* 投稿摘要： Shisa3 is a newly identified tumor suppressor gene in non-small cell lung cancer. In the study, we first screened 11 colorectal cancer (CRC) cell lines as well as 10 pairs of primary tumors and matched normal colon mucosas for Shisa3 expression by RT-PCR. Shisa3 gene was silenced or down-regulated in 8 (72.7%) cell lines and 8 (80%) tumors, suggesting that Shisa3 might be also involved in colorectal tumorigenesis. Accordingly, we further confirmed Shisa3 expression was significantly decreased in 59.6% (31/52) of colorectal carcinomas compared with corresponding normal mucosas by quantitative RT-PCR. Notably, Shisa3 mRNA expression was restored in 66.7% (4/6) of CRC cell lines as exposure to DNA methylation inhibitor 5-Aza-2’-deoxycytidine. Aberrant methylation of 5 CpG loci in Shisa3 intron 1 was identified in CRC cell lines and primary tumors by bisulfite sequencing, and their methylation levels determined by pyrosequencing were inverse correlation with Shisa3 mRNA expression. In addition, among 127 pairs of CRC tissues, Shisa3 methylation levels in tumors were much higher than that in corresponding normal mucosas (P<0.001), and Shisa3 hypermethylation was significantly associated with disease recurrence in stages B and C patients (P=0.008). Kaplan-Meier survival analysis showed that patients with Shisa3 hypermethylation revealed significantly shortened overall survival (P<0.001) and disease-free survival (P=0.006). In conclusion, Shisa3 DNA hypermethylation could serve as a molecular predictor of disease recurrence and poor prognosis in CRC.
*關鍵字1 ： Colorectal cancer
*關鍵字2 ： Shisa3
*關鍵字3 ： Tumor suppressor gene
*關鍵字4 ： DNA hypermethylation
*關鍵字5 ： Prognostic biomarker
* 服務機關：
* 第一作者： 陳紋綺
* 身分字號： *****19406
其他投稿作者： 楊雅倩
身分字號： *****41825
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 開啟檔案
審查委員意見： 同意
審查委員意見： 發現Shisa3基因甲基化程度可以作為大腸直腸癌的預後指標.可以建議"口頭報告".
主任委員意見：
 
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